The spore germination, protonemal development and gametophyte differentiation of Lindbergia brachyptera are observed in cultivation. The results show that the germinating time of the spore is 3 days, and the germination pattern is exosporous. When the culture is 8 days old, the germinating rate of spores is 95%. The protonemal system of Lindbergia brachyptera consists of two elements, the filamentous chloronema and the rhizoid. The initial of gametophytes are observed to have differentiated from the chloronema.